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efficient production of antifungal proteins in plants

efficient production of antifungal proteins in plants

Efficient Production of Antifungal Proteins in Plants ...

Antifungal proteins (AFPs) secreted by filamentous fungi are promising biomolecules that could be used to develop new antifungal therapies in medicine and agriculture. They are small highly stable proteins with specific potent activity against fungal pathogens. However, their exploitation requires efficient, sustainable and safe production systems.

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Efficient production of antifungal proteins in plants ...

Nov 22, 2018  Production of the antifungal protein AfpB in leaf apoplasts of N. benthamiana plants AfpB is an AFP whose gene was identified in the genome of the postharvest phytopathogenic fungus P. digitatum but that, unlike other AFPs, is not naturally produced by the fungus (Garrigues et al ., 2016 ).

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Efficient production of antifungal proteins in plants ...

Dec 06, 2018  Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus Xiaoqing Shi , 1 Teresa Cordero , 2 Sandra Garrigues , 3 Jose F. Marcos , 3 José‐Antonio Daròs , 2 and María Coca 1

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Addgene: Efficient production of antifungal proteins in ...

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M. Plant Biotech Journal (2018) Article

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Efficient production of antifungal proteins in plants ...

This work was supported by grants BIO2017-83184-R, BIO2015-68790-C2-1-R and BIO2015-68790-C2-2-R and through the 'Severo Ochoa Programme for Centres of Excellence in RD' (SEV-2015-0533) from Spanish Ministerio de Ciencia, Innovacion y Universidades (co-financed FEDER funds) and by the CERCA Programme/Generalitat de Catalunya. We thank Laia Castillo for technical support.

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Efficient production of antifungal proteins in plants ...

Antifungal proteins (AFPs) secreted by filamentous fungi are promising biomolecules that could be used to develop new antifungal therapies in medicine and agriculture. They are small highly stable proteins with specific potent activity against fungal pathogens. However, their exploitation requires efficient, sustainable and safe production systems.

get price

Efficient production of antifungal proteins in plants ...

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Plant Biotechnology Journal ( IF 9.803) Pub Date : 2018-12-06, DOI: 10.1111/pbi.13038

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Addgene: Efficient production of antifungal proteins in ...

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M. Plant Biotech Journal (2018) Article

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Efficient production of antifungal proteins in plants ...

This work was supported by grants BIO2017-83184-R, BIO2015-68790-C2-1-R and BIO2015-68790-C2-2-R and through the 'Severo Ochoa Programme for Centres of Excellence in RD' (SEV-2015-0533) from Spanish Ministerio de Ciencia, Innovacion y Universidades (co-financed FEDER funds) and by the CERCA Programme/Generalitat de Catalunya. We thank Laia Castillo for technical support.

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Plants produce antifungal proteins Global Plant ...

Dec 18, 2018  Phys.Org Plants as efficient antifungal factories December 11, 2018, Asociacion RUVID Nicotiana plants. Credit: Asociación RUVIDResearchers report that they can efficiently produce antifungal proteins in plants based on a modified tobacco mosaic virus. The results of this research, which could have a major impact in the agri-food industry, have been published in the Plant

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New antifungal proteins in plant biofactories

May 31, 2019  New antifungal proteins in plant biofactories. Shi X, Cordero T, Garrigues S, et al. 2018.Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus.Plant Biotechnology Journal, DOI: 10.1111/pbi.13038

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Plants as antifungal factories -- ScienceDaily

Dec 10, 2018  Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Plant Biotechnology Journal ,

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Plant antifungal proteins and their applications in ...

May 15, 2015  Plants have developed various mechanisms to defend themselves against these fungi which include the production of low-molecular-weight secondary metabolites and proteins and peptides with antifungal activity. In this review, families of plant antifungal proteins (AFPs) including defensins, lectins, and several others will be summarized.

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Antifungal Protein - an overview ScienceDirect Topics

J.H. Wong, T.B. Ng, in Comprehensive Biotechnology (Second Edition), 2011 Abstract. Antifungal proteins are produced by a variety of organisms including plants, fungi, vertebrates, and invertebrates. They are used to protect these organisms from attack by pathogenic fungi. They are a subject of intense investigations because fungal invasion of agricultural crops and farm animals can increase ...

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Therapeutic recombinant protein production in plants ...

Nov 28, 2019  Therapeutic protein production in plants is an area of great potential for increasing and improving the production of proteins for the treatment or prevention of disease in humans and other animals. There are a number of key benefits of this technique for scientists and society, as well as regulatory challenges that need to be overcome by ...

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Biotechnological Production of the Cell Penetrating ...

This study reports the efficient production of PAF102 in P. pastoris when fused to the rice plant Oleosin 18, whereas no accumulation of Cecropin A was detected. The Ole18-PAF102 fusion protein targets the lipid droplets of the heterologous system where it accumulates to high levels. Interestingly, the production of this fusion protein induces ...

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Plants as antifungal factories CRAG Centre for ...

Researchers from the Spanish Research Council (CSIC) at the Centre for Research in Agricultural Genomics (CRAG) and the Institute for Plant Molecular and Cellular Biology (IBMCP), in collaboration with the IATA, have developed a biotechnological tool to produce, in a very efficient manner, antifungal proteins in plants.The results of this research, that could impact the agri-food and ...

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Efficient production and characterization of the novel and ...

Nov 07, 2017  Garrigues, S., Gandía, M., Popa, C. et al. Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum. Sci Rep 7, 14663 (2017 ...

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Use of a PTGS-MAR expression system for efficient in ...

Use of a PTGS-MAR expression system for efficient in planta production of bioactive Arabidopsis thaliana plant defensins. Sels J(1), Delauré SL, Aerts AM, Proost P, Cammue BP, De Bolle MF. Author information: (1)Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, Heverlee, Belgium.

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Efficient production of antifungal proteins in plants ...

Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Plant Biotechnology Journal ( IF 9.803) Pub Date : 2018-12-06, DOI: 10.1111/pbi.13038

get price

Efficient production of antifungal proteins in plants ...

Antifungal proteins (AFPs) secreted by filamentous fungi are promising biomolecules that could be used to develop new antifungal therapies in medicine and agriculture. They are small highly stable proteins with specific potent activity against fungal pathogens. However, their exploitation requires efficient, sustainable and safe production systems.

get price

Efficient production and characterization of the novel and ...

Nov 07, 2017  Garrigues, S., Gandía, M., Popa, C. et al. Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digitatum. Sci Rep 7, 14663 (2017 ...

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Plants as antifungal factories CRAG Centre for ...

Researchers from the Spanish Research Council (CSIC) at the Centre for Research in Agricultural Genomics (CRAG) and the Institute for Plant Molecular and Cellular Biology (IBMCP), in collaboration with the IATA, have developed a biotechnological tool to produce, in a very efficient manner, antifungal proteins in plants.The results of this research, that could impact the agri-food and ...

get price

Production of a defensin-like antifungal protein NFAP from ...

Nov 19, 2013  Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus. Shi X, Cordero T, Garrigues S, Marcos JF, Daròs JA, Coca M. Plant Biotechnol J, 17(6):1069-1080, 06 Dec 2018 Cited

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Plants as antifungal factories EurekAlert! Science News

Dec 10, 2018  Researchers from three research institutes in Spain have developed a biotechnological tool to produce, in a very efficient manner, antifungal proteins in the leaves of the plant

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Plants as Anti-fungal Factories - Infection Control Today

Dec 11, 2018  Coca's research group, in collaboration with the IATA's researcher Jose F. Marcos, aims to develop new antifungal compounds based on the antifungal proteins (AFPs) secreted by filamentous fungi. The problem is that the synthesis of these compounds is extremely complex; hence their exploitation requires efficient, sustainable and safe production ...

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Production of Transgenic Soybean Plants with Two Anti ...

The identification of transgenic soybean plants that co-integrated the two anti-fungal protein genes was determined by polymerase chain reaction (PCR) and Southern blot analysis. Protein detection from the soybean leaves demonstrated the expression of the chitinase (CHI) and the ribosome-inactivating protein (RIP) in the six R 0 transformants.

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Antifungal Proteins Applied and Environmental Microbiology

Google Scholar. 93. Landon C., Pajon A., Vovelle F., and Sodano P. (2000) The active site of drosomycin, a small insect antifungal protein, delineated by comparison with the modeled structure of Rs-AFP2, a plant antifungal protein. J.

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Pr protein - SlideShare

May 22, 2015  Plant defensins:- Occurance Properties The main groups of antimicrobial peptides found in plants are thionins, defensins and lipid transfer proteins. The name “plant defensin” was coined in 1995 by Terras and collegues. Plant defensins are small (M.W. 5kDa), basic, cysteine-rich antifungal peptides ranging from 45 to 54 amino acids, and ...

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Characterization of a new antifungal lipid transfer ...

Oct 01, 2008  Abstract. Lipid transfer proteins (LTPs) are members of the family of pathogenesis-related proteins (PR-14) that are believed to be involved in plant defense responses. In this study, a novel gene Ltp 3F1 encoding an antifungal protein from wheat (Sumai 3) was subcloned, overexpressed in Escherichia coli BL-21 (DE3) and enriched using ammonium ...

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JoF Free Full-Text Potential of Antifungal Proteins ...

Penicillium phytopathogenic species provoke severe postharvest disease and economic losses. Penicillium expansum is the main pome fruit phytopathogen while Penicillium digitatum and Penicillium italicum cause citrus green and blue mold, respectively. Control strategies rely on the use of synthetic fungicides, but the appearance of resistant strains and safety concerns have led to the search ...

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Alternative protein sources key to future food production ...

Sep 30, 2020  Tapping protein potential . The project will address these concerns by validating innovative, cost-effective and resource-efficient healthy plant proteins (fava bean, lentil, chickpea, quinoa) and microbial biomass proteins (food-grade yeast and fungi) for the production of ingredients and products for direct human consumption.

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The nuclear magnetic resonance solution structure of the ...

Dec 16, 2014  Besides their antifungal activity, some PDF such as Arabidopsis halleri PDF1.1b confer zinc tolerance in plants. Here we present (i) an efficient protocol for the production of AhPDF1.1b by solid-phase peptide synthesis followed by controlled oxidative folding to obtain the highly pure native form of the defensin and (ii) the three-dimensional ...

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